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Image Search Results
Journal: PLoS ONE
Article Title: Evaluation of different combination of pam2CSK4, poly (I:C) and imiquimod enhance immune responses to H9N2 avian influenza antigen in dendritic cells and duck
doi: 10.1371/journal.pone.0271746
Figure Lengend Snippet: Spleen samples were collected from vaccinated duck at 21 dpv. The mRNA levels of cytokines (IL-2, IFN-α, IFN-γ, IL-6 and TNF-α) (A) and immune-related genes (MHC-I, MHC-II, CD4, CD8 and BAFF) (B) were analyzed by qPCR. Expression of target genes was normalized to those of duck β-Actin. The relative fold change was calculated using PBS injected duck as control. Data were indicated as mean ±SEM. Different letters (a~f) mean significant difference ( P <0.05). If there is one same letter, for example, ab means has no significant difference between adjacent a or b ( P >0.05).
Article Snippet: The duck spleen lymphocyte was separated and then incubated with mouse anti-duck CD4 or
Techniques: Expressing, Injection, Control
Journal: PLoS ONE
Article Title: Evaluation of different combination of pam2CSK4, poly (I:C) and imiquimod enhance immune responses to H9N2 avian influenza antigen in dendritic cells and duck
doi: 10.1371/journal.pone.0271746
Figure Lengend Snippet: Splenic lymphocytes were isolated from the spleen of vaccinated duck at 21 dpv. Splenic lymphocyte was subjected to flow cytometry to assess the percentages of CD4 + (A) and CD8 + (B) T lymphocytes. (C) Data represent the mean ±SEM of three independent experiments. Different letters (a~c) mean significant difference ( P < 0.05).
Article Snippet: The duck spleen lymphocyte was separated and then incubated with mouse anti-duck CD4 or
Techniques: Isolation, Flow Cytometry